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anti redd1  (Proteintech)


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    Structured Review

    Proteintech anti redd1
    Anti Redd1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+redd1/REDD1+specific+Polyclonal+antibody/bio_rxiv__64898__2026__03__16__710895-75-5-6
    Average 94 stars, based on 172 article reviews
    anti redd1 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Saline:

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM TrisCl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), antiDICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS ARTICLE IN PRESS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti- -actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti- -tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRPconjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRPconjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM Tris- Cl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), anti- DICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr389, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti--actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti--tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRP- conjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRP- conjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Western Blot:

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfatepolyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM TrisCl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), antiDICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS ARTICLE IN PRESS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti- -actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti- -tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRPconjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRPconjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Article Title: The REDD1-NF-κB-miRNAs-eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertension.
    Article Snippet: The resulting immunoprecipitates and cell lysates (30−40 μg protein) were separated by sodium dodecyl sulfate- polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (#GE10600023; Cytiva, Marlborough, MA, USA). .. The membranes were blocked with 5% bovine serum albumin (BSA, #BSAS0.1; Bovogen Biologicals Pty Ltd., Australia) in Tris-buffered saline (50 mM Tris- Cl pH 7.5, 150 mM NaCl) containing 0.05% Tween-20 and analyzed using immunoblotting with the respective primary antibodies: ant-eNOS (1:1000, #PA1-037, RRID: AB_325774; Invitrogen), anti- DICER (1:1000, #5362, RRID: AB_2093073; Cell Signaling Technology), anti-IKKβ (1:1000, #2684, RRID: AB_2122298; Cell Signaling Technology), anti-REDD1 (1:1000, #10638-1-AP, RRID: AB_2245711; Proteintech), anti-p65 (1:1000, #8242T, RRID: AB_10859369; Cell Signaling Technology), anti-p53 (1:1000, #2524, RRID: AB_331743; Cell Signaling Technology), anti-acetyl-p53 (1:1000, #2570, RRID: AB_823591; Cell Signaling Technology), anti-p21 (1:1000, #SC-6246, RRID: AB_823591; Santa Cruz, Santa Cruz, CA, USA), anti-p16 (1:1000, #SC-1661, RRID: AB_628067; Santa Cruz), anti-SIRT1 (1:1000, #8469S, RRID: AB_10999470; Cell Signaling Technology), anti-ICAM-1 (1:1000, #MA5407, AR TI CL E IN P RE SS RRID: AB_223596; Invitrogen), anti-VCAM-1 (1:1000, #MA5-31965, RRID: AB_2809259; Invitrogen), anti-Raptor (1:1000, #2280, RRID: AB_561245; Cell Signaling Technology), anti-phospho-S6K (Thr389, 1:1000, #9234, RRID: AB_2269803; Cell Signaling Technology), anti-S6K (1:1000, #34475, RRID: AB_2943679; Cell Signaling Technology), anti-phospho-4E-BP1 (Thr37/46, 1:1000, #9459, RRID: AB_330985; Cell Signaling Technology), anti-4E-BP1 (1:1000, #9452, RRID: AB_331692; Cell Signaling Technology), anti--actin (1:5000, #A5441, RRID: AB_476744; Sigma-Aldrich), anti-TXNIP (1:1000, #14715, RRID: AB_2714178, Cell Signaling Technology), or anti--tubulin (1:10000, #2144S, RRID: AB_2210548; Cell Signaling Technology). .. The membranes were incubated with a HRP- conjugated goat anti-mouse (1:4000, #31430, RRID: AB_228307; Thermo-Fisher Scientific) or HRP- conjugated goat anti-rabbit IgG antibody (1:4000, #31460, RRID: AB_228341; Thermo-Fisher Scientific).

    Control:

    Article Title: Arginine depletion potentiates standard-of-care chemo-immunotherapy in preclinical models of high-risk neuroblastoma
    Article Snippet: Reagents included amino acids, temozolomide, chloroquine diphosphate salt, crystal violet, resazurin sodium salt, formic acid, perchloric acid, ethyl acetate, benzoyl chloride, sodium chloride, sodium hydroxide, and acetonitrile (Sigma-Aldrich, St Louis, MO); BCT-100 (Bio-Cancer Treatment International Limited, Hong Kong), ADI-PEG 20 (pegylated arginine deiminase, Polaris Pharmaceuticals Incorporated, San Diego, CA), arginine-free SILAC DMEM and RPMI-1640, and dialyzed fetal calf serum (Thermo Fisher Scientific, Scoresby, VIC, Australia), SN-38 (Cayman Chemical, Ann Arbor, MI), irinotecan (Accord Healthcare, Durham, NC), and mafosfamide sodium salt diastereomer mixture (Toronto Research Chemicals, North York, ON, Canada). .. Antibodies included: anti-human ganglioside GD2 monoclonal 14G2a (BE0318) and mouse IgG2a isotype control C1.18.4 (BE0085) (Bio X Cell, Lebanon, NH); anti-ARG2 D9J1N (55003), anti-ASS1 D404B (70720), anti-phospho-eIF2α (Ser 51) (9721), anti-eIF2α D7D3 (5324), anti-GAPDH 14C10 (2118), anti-LC3-A/B D3U4C (12741), anti-phospho-S6 (Ser240/244) D68F8 (5364), anti-S6 5G10 (2217), and anti-SLC7A11 D2M7A (12691) (Cell Signaling Technology, Danvers, MA); anti-ASL (ab97370), anti-GAPDH 6C5 (ab8245), anti-ODC1 (ab97395), and anti-OTC (ab244243) (Abcam, Cambridge, UK); anti-ATF4 (10835-1-AP), anti-REDD1 (10638-1-AP), and anti-ASNS (14681-1-AP) (Proteintech, Rosemont, IL); anti-MYCN B8.4.B (sc-53993, Santa Cruz Biotechnology, Dallas, TX); anti-puromycin 12D10 (MABE343; Merck, Darmstadt, Germany); anti-SLC7A1 (PA5-90039, Invitrogen, Waltham, MA); HRP-conjugated goat anti-rabbit IgG (VWR International, Murarrie, QLD, Australia); and HRP-conjugated sheep anti-mouse IgG (GE Healthcare, Rydalmere, NSW, Australia). ..

    Membrane:

    Article Title: Functional remodeling of iNKT cells by sulfatide-reactive type II NKT cells reprograms alveolar macrophages to alleviate lung ischemia-reperfusion injury.
    Article Snippet: .. The membrane was blocked and incubated with primary antibodies, including anti-β-actin (1:5000, Abclonal), anti-REDD1 (1:2000, Proteintech), anti-Arid3a (1:1000, Proteintech), and anti-Arg-1 (1:5000, Proteintech). .. After primary antibody incubation, the membrane was treated with an HRPconjugated anti-mouse IgG (1:10000, Abclonal) or HRP-conjugated anti-rabbit IgG (1:10000, Proteintech).

    Incubation:

    Article Title: Functional remodeling of iNKT cells by sulfatide-reactive type II NKT cells reprograms alveolar macrophages to alleviate lung ischemia-reperfusion injury.
    Article Snippet: .. The membrane was blocked and incubated with primary antibodies, including anti-β-actin (1:5000, Abclonal), anti-REDD1 (1:2000, Proteintech), anti-Arid3a (1:1000, Proteintech), and anti-Arg-1 (1:5000, Proteintech). .. After primary antibody incubation, the membrane was treated with an HRPconjugated anti-mouse IgG (1:10000, Abclonal) or HRP-conjugated anti-rabbit IgG (1:10000, Proteintech).



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